Review



human cytokine array kit  (R&D Systems)


Bioz Verified Symbol R&D Systems is a verified supplier
Bioz Manufacturer Symbol R&D Systems manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    R&D Systems human cytokine array kit
    Effects of metformin on <t>cytokine</t> expression and ERK signaling <t>in</t> <t>RPMI</t> 2650 cancer cells in the presence of leptin. (A) Comparative cytokine array analysis of RPMI 2650 cancer cells treated with 10 ng/mL leptin alone and in combination with 10 mM metformin. 1=IL-2, 2=Serpin E1/PAI-1, 3=IL-18/IL-1FA. (B) Quantification of pERK/ERK expression levels in RPMI 2650 cancer cells treated with 10 ng/mL leptin and 10 ng/mL leptin+10 mM metformin. The expression of pERK and ERK was normalized to GAPDH expression. One-way ANOVA was performed (n=3). Data are presented as the group mean±standard deviation. ** P <0.01, *** P <0.005, and **** P <0.001. ERK, extracellular signal-regulated kinase; pERK, phosphorylated ERK; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; IL, interleukin; PAI-1, plasminogen activator inhibitor-1.
    Human Cytokine Array Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/high+sensitivity+array/Human+Magnetic+Luminex%C2%AE+Performance+Assay+Base+Kit%2C+High+Sensitivity+Cytokine+A/pmc12399906-91-11-15
    Average 93 stars, based on 17 article reviews
    human cytokine array kit - by Bioz Stars, 2026-09
    93/100 stars

    Images

    1) Product Images from "Antitumor Effects of Metformin in Squamous Cell Carcinoma under Leptin Treatment Conditions"

    Article Title: Antitumor Effects of Metformin in Squamous Cell Carcinoma under Leptin Treatment Conditions

    Journal: Preventive Nutrition and Food Science

    doi: 10.3746/pnf.2025.30.4.312

    Effects of metformin on cytokine expression and ERK signaling in RPMI 2650 cancer cells in the presence of leptin. (A) Comparative cytokine array analysis of RPMI 2650 cancer cells treated with 10 ng/mL leptin alone and in combination with 10 mM metformin. 1=IL-2, 2=Serpin E1/PAI-1, 3=IL-18/IL-1FA. (B) Quantification of pERK/ERK expression levels in RPMI 2650 cancer cells treated with 10 ng/mL leptin and 10 ng/mL leptin+10 mM metformin. The expression of pERK and ERK was normalized to GAPDH expression. One-way ANOVA was performed (n=3). Data are presented as the group mean±standard deviation. ** P <0.01, *** P <0.005, and **** P <0.001. ERK, extracellular signal-regulated kinase; pERK, phosphorylated ERK; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; IL, interleukin; PAI-1, plasminogen activator inhibitor-1.
    Figure Legend Snippet: Effects of metformin on cytokine expression and ERK signaling in RPMI 2650 cancer cells in the presence of leptin. (A) Comparative cytokine array analysis of RPMI 2650 cancer cells treated with 10 ng/mL leptin alone and in combination with 10 mM metformin. 1=IL-2, 2=Serpin E1/PAI-1, 3=IL-18/IL-1FA. (B) Quantification of pERK/ERK expression levels in RPMI 2650 cancer cells treated with 10 ng/mL leptin and 10 ng/mL leptin+10 mM metformin. The expression of pERK and ERK was normalized to GAPDH expression. One-way ANOVA was performed (n=3). Data are presented as the group mean±standard deviation. ** P <0.01, *** P <0.005, and **** P <0.001. ERK, extracellular signal-regulated kinase; pERK, phosphorylated ERK; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; IL, interleukin; PAI-1, plasminogen activator inhibitor-1.

    Techniques Used: Expressing, Standard Deviation

    Related Articles

    Cell Culture:

    Article Title: Human umbilical cord mesenchymal stem cell therapy for atopic dermatitis through inhibition of neutrophil chemotaxis
    Article Snippet: After washing, the membranes were incubated with secondary antibodies (1:2000, Invitrogen, Carlsbad, CA, USA) at room temperature for 2 h. Finally, blots were visualized using an enhanced chemiluminescence (ECL) system (Beyotime, Nanjing, China). .. HaCaT cells were cultured in 6-well plates and exposed to TNF-α/IFN-γ (10 ng/mL) or hUC-MSC-CM (50%, v/v) for 48 h. The resulting supernatant from each group was collected, and chemokine expression levels in the supernatant were determined using the Human Cytokine Array Kit (R&D Systems, Minneapolis, MN, USA) according to the instructions of the manufacturer. .. HaCaT cells were cultured in 6-well plates and exposed to TNF-α/IFN-γ (10 ng/mL) or hUC-MSCs-CM (50%, v/v) for 48 h. The culture medium was collected and centrifuged at 1000 g for 20 min. ELISA kits (Elabscience, Wuhan, China) were used to measure the concentrations of CCL5 and CXCL11 according to the instructions of the manufacturer.

    Expressing:

    Article Title: Human umbilical cord mesenchymal stem cell therapy for atopic dermatitis through inhibition of neutrophil chemotaxis
    Article Snippet: After washing, the membranes were incubated with secondary antibodies (1:2000, Invitrogen, Carlsbad, CA, USA) at room temperature for 2 h. Finally, blots were visualized using an enhanced chemiluminescence (ECL) system (Beyotime, Nanjing, China). .. HaCaT cells were cultured in 6-well plates and exposed to TNF-α/IFN-γ (10 ng/mL) or hUC-MSC-CM (50%, v/v) for 48 h. The resulting supernatant from each group was collected, and chemokine expression levels in the supernatant were determined using the Human Cytokine Array Kit (R&D Systems, Minneapolis, MN, USA) according to the instructions of the manufacturer. .. HaCaT cells were cultured in 6-well plates and exposed to TNF-α/IFN-γ (10 ng/mL) or hUC-MSCs-CM (50%, v/v) for 48 h. The culture medium was collected and centrifuged at 1000 g for 20 min. ELISA kits (Elabscience, Wuhan, China) were used to measure the concentrations of CCL5 and CXCL11 according to the instructions of the manufacturer.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Human articular chondrocytes produce IL-7 and respond to IL-7 with increased production of matrix metalloproteinase-13
    Article Snippet: Recombinant human proteins (IL-6, soluble IL-6 receptor, IL-1β, and IL-7) were purchased from R&D Systems (Minneapolis, MN, USA). .. Human MMP-13 ELISA, Human IL-7 Quantikine High Sensitivity ELISA Kit, and Human IL-7 Biotinylated Fluorokine Kit were also from R&D Systems. .. Phospho-PYK-2 antibody was from BioSource (Camarillo, CA, USA).



    Similar Products

    95
    Randox high sensitivity cytokine biochip array 1
    High Sensitivity Cytokine Biochip Array 1, supplied by Randox, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/high+sensitivity+array/CYTOKINE+ARRAY/pmc12799373-190-23-22
    Average 95 stars, based on 1 article reviews
    high sensitivity cytokine biochip array 1 - by Bioz Stars, 2026-09
    95/100 stars
      Buy from Supplier

    90
    Eve Technologies Corporation human high sensitivity t-cell discovery array 14-plex (hdhstc14
    Human High Sensitivity T Cell Discovery Array 14 Plex (Hdhstc14, supplied by Eve Technologies Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/high+sensitivity+array/human+high+sensitivity+t+cell+discovery+array+14+plex++hdhstc14/nct05806385-145-18-10
    Average 90 stars, based on 1 article reviews
    human high sensitivity t-cell discovery array 14-plex (hdhstc14 - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    94
    Bruker Corporation high sensitivity rodent brain cryo array surface coil
    High Sensitivity Rodent Brain Cryo Array Surface Coil, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/high+sensitivity+array/Surface+and+Volume+Array+Coils/pmc12989143-138-14-20
    Average 94 stars, based on 1 article reviews
    high sensitivity rodent brain cryo array surface coil - by Bioz Stars, 2026-09
    94/100 stars
      Buy from Supplier

    98
    Dectris AG sensitivity hybrid pixel array eiger2 4
    Sensitivity Hybrid Pixel Array Eiger2 4, supplied by Dectris AG, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/high+sensitivity+array/eiger2/pm41352399-154-9-15
    Average 98 stars, based on 1 article reviews
    sensitivity hybrid pixel array eiger2 4 - by Bioz Stars, 2026-09
    98/100 stars
      Buy from Supplier

    95
    Randox high sensitivity cytokine biochip array
    High Sensitivity Cytokine Biochip Array, supplied by Randox, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/high+sensitivity+array/CYTOKINE+ARRAY/pm40975239-125-25-24
    Average 95 stars, based on 1 article reviews
    high sensitivity cytokine biochip array - by Bioz Stars, 2026-09
    95/100 stars
      Buy from Supplier

    86
    Cell Signaling Technology Inc high sensitivity cytokine custom array
    High Sensitivity Cytokine Custom Array, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/high+sensitivity+array/pm40900676-54-25-31
    Average 86 stars, based on 1 article reviews
    high sensitivity cytokine custom array - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    93
    R&D Systems human cytokine array kit
    Effects of metformin on <t>cytokine</t> expression and ERK signaling <t>in</t> <t>RPMI</t> 2650 cancer cells in the presence of leptin. (A) Comparative cytokine array analysis of RPMI 2650 cancer cells treated with 10 ng/mL leptin alone and in combination with 10 mM metformin. 1=IL-2, 2=Serpin E1/PAI-1, 3=IL-18/IL-1FA. (B) Quantification of pERK/ERK expression levels in RPMI 2650 cancer cells treated with 10 ng/mL leptin and 10 ng/mL leptin+10 mM metformin. The expression of pERK and ERK was normalized to GAPDH expression. One-way ANOVA was performed (n=3). Data are presented as the group mean±standard deviation. ** P <0.01, *** P <0.005, and **** P <0.001. ERK, extracellular signal-regulated kinase; pERK, phosphorylated ERK; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; IL, interleukin; PAI-1, plasminogen activator inhibitor-1.
    Human Cytokine Array Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/high+sensitivity+array/Human+Magnetic+Luminex%C2%AE+Performance+Assay+Base+Kit%2C+High+Sensitivity+Cytokine+A/pmc12399906-91-11-15
    Average 93 stars, based on 1 article reviews
    human cytokine array kit - by Bioz Stars, 2026-09
    93/100 stars
      Buy from Supplier

    90
    Millipore high sensitivity t-cell discovery array 3-plex
    Effects of metformin on <t>cytokine</t> expression and ERK signaling <t>in</t> <t>RPMI</t> 2650 cancer cells in the presence of leptin. (A) Comparative cytokine array analysis of RPMI 2650 cancer cells treated with 10 ng/mL leptin alone and in combination with 10 mM metformin. 1=IL-2, 2=Serpin E1/PAI-1, 3=IL-18/IL-1FA. (B) Quantification of pERK/ERK expression levels in RPMI 2650 cancer cells treated with 10 ng/mL leptin and 10 ng/mL leptin+10 mM metformin. The expression of pERK and ERK was normalized to GAPDH expression. One-way ANOVA was performed (n=3). Data are presented as the group mean±standard deviation. ** P <0.01, *** P <0.005, and **** P <0.001. ERK, extracellular signal-regulated kinase; pERK, phosphorylated ERK; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; IL, interleukin; PAI-1, plasminogen activator inhibitor-1.
    High Sensitivity T Cell Discovery Array 3 Plex, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/high+sensitivity+array/high+sensitivity+t+cell+discovery+array+3+plex/pmc12183797-347-16-22
    Average 90 stars, based on 1 article reviews
    high sensitivity t-cell discovery array 3-plex - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    96
    Quanterix sensitivity single molecule array simoa nf light v2 advantage kit
    Effects of metformin on <t>cytokine</t> expression and ERK signaling <t>in</t> <t>RPMI</t> 2650 cancer cells in the presence of leptin. (A) Comparative cytokine array analysis of RPMI 2650 cancer cells treated with 10 ng/mL leptin alone and in combination with 10 mM metformin. 1=IL-2, 2=Serpin E1/PAI-1, 3=IL-18/IL-1FA. (B) Quantification of pERK/ERK expression levels in RPMI 2650 cancer cells treated with 10 ng/mL leptin and 10 ng/mL leptin+10 mM metformin. The expression of pERK and ERK was normalized to GAPDH expression. One-way ANOVA was performed (n=3). Data are presented as the group mean±standard deviation. ** P <0.01, *** P <0.005, and **** P <0.001. ERK, extracellular signal-regulated kinase; pERK, phosphorylated ERK; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; IL, interleukin; PAI-1, plasminogen activator inhibitor-1.
    Sensitivity Single Molecule Array Simoa Nf Light V2 Advantage Kit, supplied by Quanterix, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/high+sensitivity+array/NF-Light+v2+Advantage+Kit/pmc12006663-80-20-30
    Average 96 stars, based on 1 article reviews
    sensitivity single molecule array simoa nf light v2 advantage kit - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    Image Search Results


    Effects of metformin on cytokine expression and ERK signaling in RPMI 2650 cancer cells in the presence of leptin. (A) Comparative cytokine array analysis of RPMI 2650 cancer cells treated with 10 ng/mL leptin alone and in combination with 10 mM metformin. 1=IL-2, 2=Serpin E1/PAI-1, 3=IL-18/IL-1FA. (B) Quantification of pERK/ERK expression levels in RPMI 2650 cancer cells treated with 10 ng/mL leptin and 10 ng/mL leptin+10 mM metformin. The expression of pERK and ERK was normalized to GAPDH expression. One-way ANOVA was performed (n=3). Data are presented as the group mean±standard deviation. ** P <0.01, *** P <0.005, and **** P <0.001. ERK, extracellular signal-regulated kinase; pERK, phosphorylated ERK; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; IL, interleukin; PAI-1, plasminogen activator inhibitor-1.

    Journal: Preventive Nutrition and Food Science

    Article Title: Antitumor Effects of Metformin in Squamous Cell Carcinoma under Leptin Treatment Conditions

    doi: 10.3746/pnf.2025.30.4.312

    Figure Lengend Snippet: Effects of metformin on cytokine expression and ERK signaling in RPMI 2650 cancer cells in the presence of leptin. (A) Comparative cytokine array analysis of RPMI 2650 cancer cells treated with 10 ng/mL leptin alone and in combination with 10 mM metformin. 1=IL-2, 2=Serpin E1/PAI-1, 3=IL-18/IL-1FA. (B) Quantification of pERK/ERK expression levels in RPMI 2650 cancer cells treated with 10 ng/mL leptin and 10 ng/mL leptin+10 mM metformin. The expression of pERK and ERK was normalized to GAPDH expression. One-way ANOVA was performed (n=3). Data are presented as the group mean±standard deviation. ** P <0.01, *** P <0.005, and **** P <0.001. ERK, extracellular signal-regulated kinase; pERK, phosphorylated ERK; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; IL, interleukin; PAI-1, plasminogen activator inhibitor-1.

    Article Snippet: The supernatant from RPMI 2650 cancer cells was used in the human cytokine array kit (R&D Systems) following the manufacturer’s instructions.

    Techniques: Expressing, Standard Deviation